Ral G-proteins cycle between a GDP-bound “off” state and a GTP-bound “on” state in response to an incoming extracellular signal. RalGEFs (Ral guanine nucleotide exchange factors) promote the GTP-bound “on” state of a Ral G-protein by stimulating an open confirmation in guanyl nucleotide-binding pocket, which facilitates the exchange of GDP for more abundant GTP. Interaction of RalGAPs (Ral GTPase-activating proteins) facilitates a catalytically active state, which increases the rate of GTP to GDP hydrolysis to promote the “off” state. Let us assume that you are working with a cell model that has only one RalGEF protein and one RalGAP. You also observe that your Ral G-protein, let’s say CA452, is mostly “on” state even in the absence of an extracellular signal. What do you think the reason(s) is and how to design an experiment to demonstrate your suggestion?