Question
The most reproducible and accurate method of determining the amount of filamentous actin (F-actin) content versus free globular-actin (G-actin) content in a cell population is
The most reproducible and accurate method of determining the amount of filamentous actin (F-actin) content versus free globular-actin (G-actin) content in a cell population is to use Western blot quantitation of F-actin and G-actin cellular fractions.
https://www.creativebiomart.net/g-actinf-actin-in-vivo-assay-biochem-kit-462876.htm
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Total protein normalization is a useful method for obtaining accurate quantitative western blotting data as housekeeping proteins can often be affected by experimental conditions The Invitrogen NoStai...
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Statistics The Art And Science Of Learning From Data
Authors: Alan Agresti, Christine A. Franklin
3rd Edition
9780321849281, 321755944, 321849280, 978-0321755940
