LIT 3. How many different genotypes are visible on your gel? 4. How many different alleles are visible on your gel? 5. How many students on your gel are heterozygous? Which student(s)? 6. If a gel lane contains only one band, what does that tell you about that student's genotype? 7. If a PCR product is 658 bp, how many D1S80 repeats are present in that DNA molecule? 8. Give two possible reasons for why a student's sample may have failed to amplify (no bands are visible on gel). B. Lane Contents Lane #1 Lane #2 Lane #3 100 bp DNA Ladder Lane #4 Lane #5 Lane #6 Lane #7 Lane #8 C. Results and Questions 1. What is the melting temperature (Tm) of the forward PCR primer? 2. What is the melting temperature (Tm) of the reverse primer? LIT 3. How many different genotypes are visible on your gel? 4. How many different alleles are visible on your gel? 5. How many students on your gel are heterozygous? Which student(s)? 6. If a gel lane contains only one band, what does that tell you about that student's genotype? 7. If a PCR product is 658 bp, how many D1S80 repeats are present in that DNA molecule? 8. Give two possible reasons for why a student's sample may have failed to amplify (no bands are visible on gel). B. Lane Contents Lane #1 Lane #2 Lane #3 100 bp DNA Ladder Lane #4 Lane #5 Lane #6 Lane #7 Lane #8 C. Results and Questions 1. What is the melting temperature (Tm) of the forward PCR primer? 2. What is the melting temperature (Tm) of the reverse primer