Why was the alkaline phosphatase solution allowed to warm to room temperature immediately before use in the enzyme assays in Practical 4? O Pre-warming the alkaline phosphatase solution to room temperature allowed the enzyme to reach the same condition used to run the assay. This prevented any reduction in temperature that would have occurred if the solution were to have been added cold. O Alkaline phosphatase is denatured on ice and needed to be brought to an active state. O All options shown are correct. The assay would not have shown any colour change for the 3 min period if alkaline phosphatase solution was added cold. Alkaline phosphatase is unable to bind substrate at temperatures below 25 C