Lysozyme is the enzyme cleaving peptidoglycan(copolymer of N-acetylglucosamine(GlcNAc) and N-acetylmuramic acid(Mur2Ac)) polymer with Beta(1→4) linkage. Its active site has two key carboxylated amino acid residues such as glutamate(E) and Aspartate(D). Assuming the reaction starts from glutamate, there are two reaction mechanisms, i.e. SN1 and SN2 reaction mechanism, The SN2 mechanism occurs by the interplay between the two residues(E and D), while SN1 mechanism occurs by using only E and generating glycosyl cabocation as an intermediate.

1. the SN1 mechanism and

2. SN2 reaction mechanism into 3 steps