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Measurement of total phosphorus in water can be achieved by acid digestion of the water sample followed by formation of a reduced phosphomolybdate species called
Measurement of total phosphorus in water can be achieved by acid digestion of the water sample followed by formation of a reduced phosphomolybdate species called molybdenum blue which is intensely coloured. The concentration is determined by measurement of absorption at 880nm. The following calibration data were used in a phosphate determination in lake water samples: absorbance at 880nm 0.000 0.001 0.100 0.122 0.250 0.304 0.500 0.612 1.000 1.216 mg P.L-1 A 10.00 mL sample of lake water and a 10.00 mL aliquot of MilliQ water (digestion blank) were each acid digested and each made up to 25.00 ml. The phosphorus in each digest was then treated to form molybdenum blue and absorbances measured. For the lakewater sample digest an absorbance of 0.228 was measured, and for the blank digest an absorbance of 0.025 was measured. Calculate the concentration of phosphorus in original lake sample in mg.PL-1. (Do not include units, do not use scientific notation in submitting your answer.)
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