mmol

An oligomeric protein has a molecular mass of 4.13 x 10 g-mol ! A 720-mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next, the peptide bonds were hydrolyzed by heating with concentrated HCl. The product of the complete hydrolysis contained 3.30 mg of dinitrophenyl-methionine. No other 2,4-dinitrophenyl derivatives were present. NO ON N COOH CH S CH An oligomeric protein has a molecular mass of 4.13 x 10 g-mol ! A 720-mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next, the peptide bonds were hydrolyzed by heating with concentrated HCl. The product of the complete hydrolysis contained 3.30 mg of dinitrophenyl-methionine. No other 2,4-dinitrophenyl derivatives were present. NO ON N COOH CH S CH