Molecular Peer Challenge

A) Nucleic Acid Amplification

Give a short pedagogical objective of each question for students.

Level I: Recall and Comprehension

What is the primary purpose of nucleic acid amplification in molecular diagnostics?

Objective:

A) To degrade RNA molecules

B) To bind antibodies to antigens

C) To increase the amount of specific DNA or RNA sequences

D) To enhance protein synthesis

Level II: Application and Interpretation

A laboratory technician is using polymerase chain reaction (PCR) to detect a viral infection. Which step is crucial for amplifying the viral DNA present in the sample?

Objective:

Level III: Evaluation and Problem Solving

A scientist is comparing the effectiveness of different nucleic acid amplification technologies for diagnosing bacterial infection in blood samples. Which aspect should be prioritized to determine the most suitable method for rapid and sensitive detection?

Objective:

B) DNA Tissue Typing

Level I: Recall and Comprehension

What is the main objective of DNA tissue typing in transplantation medicine?

Objective:

Level II: Application & Interpretation

A hospital is preparing for a kidney transplant and requires DNA tissue typing. The laboratory receives samples from the donor and recipient. What is the most important genetic marker to consider when determining compatibility?

Objective: