NA = Numerical Aperture

DOF = Depth of Field

Q4: Obtain the NAs and DOFs for (1) a 15.0 keV electron microscope (2) a 500 nm (2.48 eV) visible light optical microscope with a spatial resolution of 1.00 um. Assume diffraction limited cases and Rayleigh's criterion and use the equations on page 16. Spatial resolution 8 (Hopkins' Resolution) 8 = NA Diffraction limited 82 2: wavelength of light doc 2 Rayleigh's Resolution (Rayleigh's criterion): K = 0.61 Abbe's Resolution: K = 0.5 For a given 8, d is inversely proportional to a Depth of field (DOF) d 2 d NA2