Nalaxone is an opioid blocker critical to the treatment of opiod overdose, but its mechanism of action is not yet fully understood. Arachidonyl-29-chloroethylamide (ACEA) is a molecule designed to activate a specific cannabinoid receptor in the brain so that the receptor's function can be studied. Like cannabis, ACEA induces a hyperfeeding response in mice. Researchers randomly assigned 22 mice that had just fed to 4 treatments consisting of an injection: ACEA alone, nalaxone alone, ACEA plus nalaxone, saline only (control). They then measured the amount of food the mice consumed within 1 hour of treatment. Here are the findings (in milligrams, mg).

ACEA Naloxone ACEA + naloxone Control

130 10 10 20

150 40 50 30

150 40 60 40

190 120 80 80

250 120 120 170

140 160

What does your analysis suggest about the involvement of naloxone in cannabinoid-induced hyper feeding in mice? Use the four-step process to guide your discussion.