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Now we have an enzyme - catalyzed reaction, in which the enzyme ( E ) reacts with the substrate ( S ) to generate a

Now we have an enzyme

-

catalyzed reaction, in which the enzyme

(

)

reacts with the substrate

(S)

to generate a product.

E + S_{k_{2}}^{k_{1}} E S k_{3} E +

Product

The rate constant of each step, i

.

., k_{1}, k_{2},

and

k_{3},

is not known. How could we obtain the maximum initial reaction rate?

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