Now we have an enzyme$-$catalyzed reaction, in which the enzyme $($E$)$ reacts with the substrate $(S)$ to generate a product.

$E+S{}_{k_2}^{k_1}ES{k}_{3}E+$ Product

The rate constant of each step, i$.$e$.,k_1,k_2,$ and $k_3,$ is not known. How could we obtain the maximum initial reaction rate?