pads/6fdd 1e4ca7e08ca9244973640e220f3f00783083.pat Draw Highlight + [ Page view | A Read aloud [ Add text 1 V V Erase The key idea is to place an image of the filament at the back focal plane of the condenser, so that each point on the filament produces a parallel beam of light that uniformly illuminates the sample. See Fig. 4 for a transmitted-light version. Try this tutorial on Microscopy U. Pre-lab Question 1: If the microscope's calibration factor is 100 nm / pixel, what dilution of beads do you need to make to achieve the desired density in the viewing area? The density of polystyrene is ~ 1.05 g/ml. Assume that the depth of field ~ 5 um and that you are doing your first measurements with beads with diameter 1 um. Assume, also, that the concentration is as specified in Sec. 2.2. For 833 students: The problem here implicitly assumes that the concentration of beads is uniform. Is it? What does the Boltzmann distribution for bead concentration imply? Calculate the condition implied by the Boltzmann distribution and see whether it is relevant. Assume a chamber thickness of 100 jm