Part 1: Preparation Of A Volumetric Stock Solution Mass of methylene blue (g) 0.713 Volume of stock solution (mL) 100.0 Part 2: Pipetting And Serial Diluting Calculations Dilution 1st 2nd 3rd Volume (mL) Aliquot 10.00 10.00 10.00 Volume Diluted To 100.00 100.00 100.00 80 Part 1: Preparation Of A Volumetric Stock Solution Determine the molarity of the methylene blue stock solution. Methylene blue's molar mass is 319.85 g/mol.