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Please answer questions 2 & 3 for GFP determination! 1. Generate a figure for the 300 - 600 nm scan. 2. Calculate the concentration using

Please answer questions 2 & 3 for GFP determination!

1. Generate a figure for the 300 - 600 nm scan.

image text in transcribed 2. Calculate the concentration using the extinction coefficient (56,000 Cm^-1 M^-1) and then calculate the total amount of GFP in the original, undiluted, crude sample. (dilution 1:10 with a final volume of 200uL) 3. Calculate the relative purity (R.P.) of your crude extract supernatant.

Absorbance vs. Wavelength; lysate-buffer

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