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Please answer questions 2 & 3 for GFP determination ! 1. Generate a figure for the 300 - 600 nm scan. 2. Calculate the concentration

Please answer questions 2 & 3 for GFP determination !

1. Generate a figure for the 300 - 600 nm scan.

2. Calculate the concentration using the extinction coefficient (56,000 Cm^-1 M^-1) and then calculate the total amount of GFP in the original, undiluted, crude sample. (dilution 1:10 with final volume of 200uL 3. Calculate the relative purity (R.P.) of your crude extract supernatant.

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