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Please answer to all questions regarding microscope and include hand drawing pictures for the related questions 3- How would you place your sample and get

Please answer to all questions regarding microscope and include hand drawing pictures for the related questions

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3- How would you place your sample and get it in focus on a light microscope? List the steps. Draw a diagram illustrating the light path through a light microscope and label the key components of it. Draw a diagram illustrating the light path through a widefield microscope and label the key components of it. Dichroic mirrors and excitation filters play a critical role in the structure of fluorescence microscopes, explain their main functions. The radiative emission of excited fluorophores has longer wavelength than that of the excitation one. Mention the name of this phenomenon, why it happens? And how researchers can take advantage of it? Name types of microscopes that differ in terms of their orientation ofthe objectives relative to the stage, and describe a situation where you can use one type of microscope but not the other. Arrange the following list of objectives according to their resolution power, from strongest to weakest, and calculate the minimum resolution distance for each of these objectives. a. 10ml, NA=D.3, wavelength: 'I'Clnm b. 10x, NA=EI.2, wavelengtthUOnm c. 100x, NA=1, wavelength=?[}nm d. EUx,NA=U.8, wavelength=500nm Describe the importance of dissecting microscopes

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