please do detailed material and energy balance for the above process flow diagram. assume any values if required.

15.4.6. Production and extraction in SSF The production of Monascus in SSF is very straightforward, except for the fact that, depending on the fermentation conditions, the substrate may lose its rigidity, so that a suitable solid layer must be used (generally 2 to 15cm ). Figure 3 illustrates Monascus pigments production over rice, in trays. Figure 3. Monascus production in trays When only the coloring fraction is necessary, without substrate residues - for use in beverages, for an instance - the pigment must be extracted with an organic solvent, such as 60-70\% ethyl alcohol (pure ethanol is less efficient; methanol is very efficient, but toxic). After the extraction, the solvent is evaporated and the pigment eventually processed. (Carvalho et al., 2006). Depending on extraction conditions, an adequate filler must be used to enhance processing