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please explain 7. For many enzymes, such as chymostrypsin, the mechanism of action involves the formation of two intermediates: E +S - ES v =

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7. For many enzymes, such as chymostrypsin, the mechanism of action involves the formation of two intermediates: E +S - ES v = ka [E] [S] ES - E + S v = ka[ES] ES - ES' v = kb [ES] ES' - E + P v = kc[ES'] Show that the rate of formation of product has the same form as that shown in eqn 8.1, written as: Vmax D= - 1 + KM [Slo but with Vmax and KM given by: KokcLElo Vmax = Kb + Kc Kc (ka + kb) KM = Ka(kb + Kc)

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