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please explain the answer in detail Another study, independent of the fiest one, examined the eftect of temperature on wild-type E. coli cell growth kinetics
please explain the answer in detail
Another study, independent of the fiest one, examined the eftect of temperature on wild-type E. coli cell growth kinetics and produced the following results, plotted on the semi-log figure below (Fipure 2). The symbols represent the experimental data and the lines various mathematical models used to fit the data: lnx Figure 2. Effect of culture temgerative on cell growth knetics of wild type E. coli. Q4. Comment on the relative diflerences in growth rate or doubling time during the exponential phase. For simplification used the Fied line in both graphs for your analysis. Q5. Compare the overall specific GFP productivities obtained at the two temperatures. "Overall specific productivity" implies that one takes the entire duration of the experiment inte account (GFP/OD - 0. Assume that: 1) The E. coll cels used for the study featured in Figure 1 exhibit the same growth kinetics at 25 and 3C than that described for the study featured in Figure 2; 2) IPTG was added at the end of the exponential phase when a plateau starts (use Figure 2, as an examplel: 3) The induction phase (= the plateau) lasts 4 hours and does not lead to any cell mass increase flate can be seen in Figure 2l; and 4) Induced cells are harvested after the 4 hour induction period. 2 Q6. If the goal of the study is to maximite the overall specific productivity, which temperature would you select? Q7. If the goal of the study were to maximize the overall productivity of Green fluorescent protein (GFP/At). would your analysis reported in Q6 differ? Another study, independent of the fiest one, examined the eftect of temperature on wild-type E. coli cell growth kinetics and produced the following results, plotted on the semi-log figure below (Fipure 2). The symbols represent the experimental data and the lines various mathematical models used to fit the data: lnx Figure 2. Effect of culture temgerative on cell growth knetics of wild type E. coli. Q4. Comment on the relative diflerences in growth rate or doubling time during the exponential phase. For simplification used the Fied line in both graphs for your analysis. Q5. Compare the overall specific GFP productivities obtained at the two temperatures. "Overall specific productivity" implies that one takes the entire duration of the experiment inte account (GFP/OD - 0. Assume that: 1) The E. coll cels used for the study featured in Figure 1 exhibit the same growth kinetics at 25 and 3C than that described for the study featured in Figure 2; 2) IPTG was added at the end of the exponential phase when a plateau starts (use Figure 2, as an examplel: 3) The induction phase (= the plateau) lasts 4 hours and does not lead to any cell mass increase flate can be seen in Figure 2l; and 4) Induced cells are harvested after the 4 hour induction period. 2 Q6. If the goal of the study is to maximite the overall specific productivity, which temperature would you select? Q7. If the goal of the study were to maximize the overall productivity of Green fluorescent protein (GFP/At). would your analysis reported in Q6 differ Step by Step Solution
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