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please help with all parts H 0---H-N H N-H-0 R R NE N----H-N -N o R N-H-----N N= N-H-----0 R H Guanine Cytosine Adenine Thymine

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H 0---H-N H N-H-0 R R NE N----H-N -N o R N-H-----N N= N-H-----0 R H Guanine Cytosine Adenine Thymine A adenine T thymine G guanine cytosine 2 2 2 How many carbon rings are there in each base? Check if the following functional groups are present: NH - CEO -NH2 - CH3 Is this base a purine? Is this base a pyrimidine? The base with the -CHfunctional group is The base with the -NH, group but without the-C=0 group is A purine with the-C=O group is A pyrimidine with the -NH2 group is Part B: Investigating the differences between DNA base pairs How many hydrogen bonds hold together Adenine and Thymine? Guanine and Cytosine? A-T or G-C Which base pairs are most strongly bonded (circle)? Give a definition of a hydrogen bonding List all hydrogen bond connections of the form (base) -OH N-(base) or (base) -N H N-(base). For example: (Guanine)-O H-N-(Cytosine) A-T base pair G-C base pair What is the average distance between hydrogen bonded atoms for each base? (obtain three distances between N H or H O atoms) #2 #3 average #1 Adenine - Thymine Guanine - Cytosine The average length of a hydrogen bond (Average all distances to obtain an overall average) Compare the values of a hydrogen bond length to an average chemical bond length. Use your textb Compare the energy of a hydrogen bond to an average chemical bond. Use your textbook. Measure distances between the two furthest atoms three times using different atoms as reference points. This measurement gives you an approximate width of the helix #2 #3 average Adenine - Thymine Guanine - Cytosine The average base-pair distance (Average all six distances to obtain an overall average width) Is the horizontal distance between A and in an A-T base pair similar to that between G and in G-C? How does this influence the DNA helix structure? Part C: Examining Two Base Pairs Base Pair Rise is the average distance between the planes of the two stacked base pairs B-DNA A-DNA The rise of the helix is The helix twist is One complete turn of the helix is 360 Predict the number of base pair planes for one complete turn Given the rise for a pair of base planes, Predict the total height of a full turn Part D: Investigating the full turn of DNA duplex. CGCGAATTCGCG sequence. A-DNA B-DNA Measure: diameter of the helix height of one full turn #base pair steps per full tum Compare the height and the number of base pairs with the predictions in Part C. Compare A-DNA and B-DNA viewing down the helix axes (write a complete sentence) Post-Lab Questions What are the necessary conditions for H-bonding? What is a complementary DNA strand to ACGCTTCACT sequence? What are a specific condition for A-DNA helix for B-DNA helix

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