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Plot pH vs. % in the form of In - (Graph 3). Plot log [In - ]/[HIn] vs. pH (Graph 4). Calculate pKa for the

Plot pH vs. % in the form of In-(Graph 3).

Plot log [In-]/[HIn] vs. pH (Graph 4).

Calculate pKa for the dissociation of bromothymol blue from both plots.

pKa from graph 3 = _____________

pKa from graph 4 = _____________

Questions

Q1)

A copper sulphate solution has a molar absorptivity value of 20 L mol-1 cm-1.

Beta -carotene is an organic compound found in vegetables and is responsible for the color of carrots. Its molar absorptivity is 100,000 L mol-1 cm-1. Which compound is easier to be detected at low concentration using spectrophotometer?

Q2) Which curve represents the relationship between the light path length and absorption?

Q3) Which of the following is correct? Select all applied

If we plot absorbance against concentration, we get a straight line passing through the origin (0,0).

is a measure of the amount of light absorbed per unit concentration".

low concentrations of a compound with a high molar absorptivity can be easily detected compared to low concentrations of a compound with low molar absorptivity

Molar absorptivity is a constant for a particular substance, so if the concentration of the solution is halved so is the absorbance

molar absorptivity affects the limit of detection of a particular compound.

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