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Pls answer all parts, thank you a. (i) In the HPLC analysis of a mixture of compounds A and B, the more efficient the column
Pls answer all parts, thank you
a. (i) In the HPLC analysis of a mixture of compounds A and B, the more efficient the column the greater degree of resolution it will produce between closely eluting peaks. Draw a chromatogram showing two well separated peaks corresponding to compounds A and B. On the chromatogram indicate the retention times for A and B, the widths at baseline W for both peaks, and the column dead-time t0. (ii) Provide the equation that relates the resolution between two peaks Rs to the retention times tr and to the widths at baseline W of peaks A and B. (iii) What is the minimum value of Rs for two well-shaped, symmetric peaks to be differentiated successfully? b. A solution containing a mixture of compounds A and B is analyzed with HPLC. The retention (or capacity) factors k for compounds A and B are 6 and 10 , respectively. Knowing that the void volume of the column is V0=1 ml and that the solvent flow rate for compound A is rA=1ml/min and for compound B is rB=2ml/min, calculate the retention time tr for compounds A and BStep by Step Solution
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