Preparation of 200 mL of TRIS-ME BUFFER (10 mM Tris-HCl, pH 8.6; 0.5 mM 2-mercaptoethanol). You prepared 500 mL of 10 mM Tris-HCl, pH 8.6 on day 1. To 200 mL of this solution, add 2-mercaptoethanol to a final concentration of 0.5 mM. The 2-mercaptoethanol is in the hood (pure 2-mercaptoethanol is 14.3 M).

Before pipetting the 2-mercaptoethanol, What is the Volume of 2-mercaptoethanol needed: