Prepare 50 ml of 10X Tris-EDTA (TE; which is 100mM Tris, 10 mM EDTA) from the stock solutions prepared in A and B. (A) Prepared 250 ml of 0.5 M EDTA (292.24 g/mol). (B) Prepared 500 ml of 1 M of Tris buffer (121.14 g/mol).