Prepare a 0.15% (w/v) solution of haemoglobin in 10 mM HCl pH2. Dilute to 0.03, 0.05, 0.07% and 0.09% in 10 mM HCl pH 2. 

2. Prepare a calibration curve of haemoglobin in 10mM HCl pH 2 at 372 nm wavelength in quartz cuvette for the solutions of haemoglobin provided (0.01 -0.07 % (w/v) in 10 mM HCl pH 2 solution), using 10 mM HCl pH 2 solution as the blank for the absorbance measurement. 

a) Set your UV spectrometer to collect a spectrum from 200 nm - 700 nm 

b) Rinse your plastic cuvette with the 10 mM HCl pH 2 solution. 

c) Put the 10 mM HCl pH 2 into a plastic cuvette, place in the spectrometer and run a baseline. 

d) Empty the cuvette and rinse the cuvette with the most dilute haemoglobin solution (0.03% w/v) first before filling it with the same solution, placing in the spectrometer and collecting the spectrum. e) Analyse the spectrum to find the wavelength with the highest absorbance peak (Amax). Note the absorbance at this λmax. f)