To assist the researcher transcribe and translate each of the mitochondrial DNA fragments taken from the patient. There isn’t a start codon! Compare the resulting amino acid sequence of the patient with that of normal individuals to determine which type of mutation, if any, the protein has. Pyruvate Dehydrogenase (aka – pyruvate decarboxylase) Normal amino acid sequence: phenylalanine-proline-lysine-glycine-arginine-alanine-valine-leucine DNA sequence of patient: 3’ A A A A G G G T T C C C A G C T C G T C A C G A 5’ Pyruvate transport protein Normal amino acid sequence : serine-threonine-isoleucine-tyrosine-histidine-tryptophan-lysine-proline DNA sequence of patient: 3’ A G A T G C T A G A T G G T A A C C T T C G G C 5’ Citrate Synthetase Normal amino acid sequence : valine-alanine-serine-cysteine-cysteine-aspartate-leucine-phenylalanine DNA sequence of patient: 3’ C A G C G G A G A A C A A C C C T A G A C A A A 5’ Cytochrome oxidase Normal amino acid sequence : glutamate-histidine-tyrosine-leucine-valine-glycine-arginine-proline DNA sequence of patient : 3’ C T T G T A A T T G A T C A T C C C T C T G G G 5’