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Problem 1 . The adsorption isotherms of two proteins in an ionic exchange resin are: With k r = 0 . 2 m g of
Problem The adsorption isotherms of two proteins in an ionic exchange resin are:
With
of of adsorbent
of of liquid
of of adsorbent
of of liquid
The two proteins are separated in a chromatography column filled with of this resin. The dimensions of the gel bed are: diameter: height: If the volume of the mixture added is and the porosity of the bed is determine:
a The position of each band in the column
b The ratios between the distances travelled by each of the proteins to the distance travelled by the solvent when and
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