Problem 1: The following data were obtained for product production by an enzymatic reaction. The reaction takes place under first order reaction condition. Solve the following: 1) Determine the rate constant of the reaction 2) Estimate the product concentration after 20 hrs 3) How would the reaction rate would differ with The initial substrate and enzyme concentrations? Problem 2: The following set of data have been obtained for an enzymes-catalyzed reaction at specified enzyme concentration of 0.038mol/L. Solve for the following 1) Determine the Michaelis-Menten kinetics paramters 2) Calculate reaction rate constants (k2) 3) Determine the initial reaction rate at [S]=12molL if enzyme concentration got increased by 35%. Explain your answer 4) How would reaction rate constant would be affected if enzyme concentration increases by 35%. Explain your answer Problem 3: Interest toward using Eversar. Transform 2.0 enzyme is increasing nowadays for esters production. It catalyze the conversion of waste cooking oils to biodiesel. Given MichaelisMenten plot determine, solve for the following: 1) Specify the type of inhibition for each inhibitor 2) Determine the Michaelis-Menten kinctics parameters for the non-inhibition case and inhibitor B Problem 4: Data of Problem 3 are given below, solve for the following: 1) Determine kinetic parameters for all cases using L-B method. Comment on obtained parameters 2) Prove that KM equals [S] when the initial rate is half its maximum value