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Problem 5. Lactose intolerance is a condition in which patients are unable to efficiently digest the common dairy disaccharide lactose. As a clinical researcher, you
Problem 5. Lactose intolerance is a condition in which patients are unable to efficiently digest the common dairy disaccharide lactose. As a clinical researcher, you are examining the enzyme lactase, which facilitates the breakdown of lactose. You know that the typical kinetic constants of lactase are kcat=0.45s1 and KM=2mM and take samples from the small intestine of a lactose tolerant and lactose intolerant patient and measure the initial rates of reaction in an 0.5mM lactose solution. You obtain the follow initial rates of reaction: A. Given this information, calculate the initial concentration of lactase in the sample taken from each patient. B. For each individual, what is the theoretical maximum rate at which they can digest lactose? C. For the lactose intolerant individual only, the concentration of lactose in the small intestine after a dairy heavy meal was 72mM. If the researcher assumes that the reaction will occur constantly at the maximum rate, what percentage of lactose is digested after 1 hours
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