Question
Proteins can be extracted without denaturation into solutions of isooctane containing the detergent Aerosol OT. The proteins are solubilized within inverted micelles of detergent. Because
Proteins can be extracted without denaturation into solutions of isooctane containing the detergent Aerosol OT. The proteins are solubilized within inverted micelles of detergent. Because the amount solubilized is a strong function of pH, the proteins can be extracted into organic at one pH, often near the isoelectric point, and then later stripped at a different pH. You are studying an aqueous solution containing 0.2 vol% protein, for which at the isoelectric point,
x = 2.9y*
where x is the protein concentration in the organic phase and y* is the equilibrium concentration in the aqueous phase. You have 6.8 L of this solution and 3.8 L of organic solution.
b. What are they if you contact the solutions in three countercurrent stages?
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