Q$3$a$-3$c

Assume there was $y$ ug of lipid$-$bound $$ in the $1mL$ re$-$dissolved lipid in step $19$ of

session $1$

$500$ uL was withdrawn for Pi analysis $($step $20),$ what was the mass of Pi in $500$ uL$?$

The above sample was treated by perchloric acid, heated to dry and dissolved in $2mL$

water $($step $3,$ protocol $2,$ session II$).$ What was the mass of the Pi sample in $4g?$

Out of the $2mL$ sample, $400L$ was used for Bartlett assay $($sample $3).$ What was the

mass of $$ in the $400L$ sample $3?$ How about the masses in those diluted samples?

From the calibration curve, you can work out mass of Pi in the $400L,$ samples, known

as $dg$ in Q$3$a$.$

Then you can work backwards to find out $y$ ug from dug.