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Q1 (15 pts). Suppose we have a sample of an aqueous solution of an absorbing substance in a rectangular glass sample holder with a length
Q1 (15 pts). Suppose we have a sample of an aqueous solution of an absorbing substance in a rectangular glass sample holder with a length of 1.0cm, as shown in the following figure. The incident light has intensity, lo, equal to 100 intensity units. (Refractive index values: n (air) =1.0,n (glass) =1.53,n (aqueous solution)=1.20) a) If 100%T adjustment is first completed, then the sample absorbance is measured using UV-vis spectrometer, calculate absorbance values at I1 and I2. b) Calculate the percent reflection loss when a beam of radiant energy passes through only the first glass cell that contains aqueous solution (also absorbed 50% of the beam) as shown in the figure above
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