Q2. Assume that you have isolated an unknown protein and wish to estimate its molecular weight using column gel filtration with several standart proteins. The gel material is Sephacryl S-100. The following elution volumes were measured. Protein Molecular Weight Elution volume (ml) Hemoglobin 64,500 10.1 Egg albumin 45,000 14.5 Chymotrypsinogen 23,200 25 Myoglobin 16,900 28.2 Cyctochromec 13,000 31.0 unknown ? 7.5 What is the approximate molecular weight of the unknown protein? Hint: prepare a graph like in Figure 1 given below. 100,000 80,000 60,000 1 B 40,000 Molecular mass (daltonis T 20,000 E 10,000 Eution volume Figure 1. A plot of log molecular mass vs. elution volume for the proteins A, B, C, D and E