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Q3. A protein X has a molecular weight of 500 kDa and is negatively charged. The protein is secreted out of the cell after synthesis.

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Q3. A protein X has a molecular weight of 500 kDa and is negatively charged. The protein is secreted out of the cell after synthesis. You have a centrifuge, a nanofilter, polyethylene glycol and dextran mixture, Superdex gel filtration column and cellulose-diethylaminoethyl column. Design a downstream process for protein X using above materials. Marks: 5 *** Best of Luck ***

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