Question
Question 10 (1 point) Which of the following methods would be expected to help elute (i.e., WEAKEN the binding of) proteins from a CATION EXCHANGE
Question 10 (1 point)
Which of the following methods would be expected to help elute (i.e., WEAKEN the binding of) proteins from a CATION EXCHANGE column with carboxymethyl groups in the stationary phase and a mobile phase with pH = 7.0. Each correctly assigned choice (i.e., either checked or not) earns 0.25 marks. Each incorrectly assigned choice earns 0 marks.
Question 10 options:
|
Increasing the pH of the mobile phase from 6.0 to 7.0 for a protein with pI = 6.5.
|
|
Increasing the pH of the mobile phase from 6.0 to 7.0 AND increasing the concentration of salt (NaCl) from 0.1 M to 0.5 M for a protein with pI = 7.5.
|
|
Decreasing the pH of the mobile phase from 6.0 to 5.0 for a protein with pI = 6.5.
|
|
Increasing the concentration of salt (NaCl) in the mobile phase from 0.1 M to 0.5 M for a protein with pI = 8.0.
|
Question 8 (1 point)
Peptide A: EMDKLARP
For peptide A, there are ___ peptide bonds, and ____ functional groups can react with dansyl chloride.
Question 8 options:
|
7 ; 2
|
|
8 ; 1
|
|
8 ; 2
|
|
7 ; 1
|
Step by Step Solution
There are 3 Steps involved in it
Step: 1
Get Instant Access to Expert-Tailored Solutions
See step-by-step solutions with expert insights and AI powered tools for academic success
Step: 2
Step: 3
Ace Your Homework with AI
Get the answers you need in no time with our AI-driven, step-by-step assistance
Get Started