Question 10 (1 point)

Which of the following methods would be expected to help elute (i.e., WEAKEN the binding of) proteins from a CATION EXCHANGE column with carboxymethyl groups in the stationary phase and a mobile phase with pH = 7.0. Each correctly assigned choice (i.e., either checked or not) earns 0.25 marks. Each incorrectly assigned choice earns 0 marks.

Question 10 options:

	Increasing the pH of the mobile phase from 6.0 to 7.0 for a protein with pI = 6.5.
	Increasing the pH of the mobile phase from 6.0 to 7.0 AND increasing the concentration of salt (NaCl) from 0.1 M to 0.5 M for a protein with pI = 7.5.
	Decreasing the pH of the mobile phase from 6.0 to 5.0 for a protein with pI = 6.5.
	Increasing the concentration of salt (NaCl) in the mobile phase from 0.1 M to 0.5 M for a protein with pI = 8.0.

Question 8 (1 point)

Peptide A: EMDKLARP

For peptide A, there are ___ peptide bonds, and ____ functional groups can react with dansyl chloride.

Question 8 options:

	7 ; 2
	8 ; 1
	8 ; 2
	7 ; 1