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Question 3: (10 marks) As a biochemistry project student, you study the properties of a peptide hormone with the following sequence: (Assume the residues are

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Question 3: (10 marks) As a biochemistry project student, you study the properties of a peptide hormone with the following sequence: (Assume the residues are listed from the amino- to carboxy-terminal direction, left to right. For example, the last residue would be "T", threonine) ASNQLH VILLAWLPGVVQALLRT In the table below, list all chemical groups/ side chains that can be ionisable (ie can become charged). (8 marks for table) Include details of their pKa values, and charge at the given pH's listed Use this information to calculate net charge for this peptide for all 3 of the listed pH values. Chemical group pka / pkr value Charge: pH 1.0 Charge: pH 7.0 Charge pH 11.0 Net charge Question 3 continued: Using the information in your table: what is the isoelectric point (pl) for this peptide? Please show the calculation used to determine your answer (2 marks). Note: You answer should not exceed the space provided

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