When alkaline phosphatase is added to substrate in borate buffer (pH 9.5) and allowed to sit at room temperature for 3 min, a yellow colour is seen to develop. Select the following option(s) that describe this situation. O A highly intense yellow colour indicates a high concentration of product in solution O A highly intense yellow colour indicates a high concentration of substate in solution. Reaction catalysed by alkaline phosphatase is being monitored via disappearance of substrate. Reaction catalysed by alkaline phosphatase is being monitored via appearance of product. O Options (a) and (d) are both correct.