Question 4 (4 points) Shown below is a pairwise alignment of two sequences. The sequence in the middle is the consensus sequence. Calculate the following values for the following pairwise alignment, using PAM250 provided. Assuming a gap opening penalty of -3, and a gap extension penalty of 0.5. A R N D C Q E G H L M F P S T W Y V B Z x A 2 -2 0 -2 0 0 1 -1 2 1 1 1 6 -3 0 0 0 R 6 -1 1 32 2 EE 0 4 0 2 4 2 -1 0 -1 N 2 D C 452 24 1 1 2 .2 3 1 -2 3 0 1 0 -4 -2 -2 2 1 2 3 1 1 -2 4 0 3 6 -7 -4 2 3 3 -1 12 S -5 3 -3 2 6 -5 -5 4 3 0 -2 8 -2 4 -5 -3 4 2 31 2 2 1 1 0 -1 5 -4 -2 1 3 -1 E 4 0 1 2 -3 0 2 -5 -1 0 -7 4 2 3 3 -1 G 5 2 -4 -2 -3 -5 0 1 0 7 5 -1 0 0 -1 H 6 2 -2 0 -2 -2 0 -1 +1 3. 2 1 2 -1 T 5 2 2 2 1 -2 -1 0 5 1 4 -2 -2 -1 L 6 3 4 2 3 J -2 2 1 2 -3 3 1 x 5 0 -5 0 0 3 -4 -2 1 D -1 M 6 0 -2 2 -1 4 -2 2 -2 -2 -1 F 9 P S T W Y 56 3 -3 0 7 1 4 -5 2 1 0 2 1 62 -5 - -1 0 -1 -3 +1 3 5 -3 17 10 V 4640 105 0 0 1 0 -5 6 4 2 B 3 323 -3 4 2 2 -1 2 -1 Z 3 5 x -1