question 5th please

question 5th ammonium sulfate precipitaion of LDH lab

we molanty of ammonium sufate at this step. 4. Why did we switch from using phosphate to using bicine as a buffer? Hint: what are the pka of these buffers? Brielly explain What is the structure of bicine? What is the pH of an ammonium sulfate solution? Does the phosphate buffer have much effect on pH once the ammonium sulfate is added? 5. Suppose you have protein dissolved in 50.0 ml total volume and that this solution is also exactly 1 Min NaCl. You then dalyze against 950 m of 50 mm phosphate until equilibrium is reached and transfer the sample, still in tubing, to a new 950 ml batch of 50 mM phosphate and dialyze again to equilibrium Calculate the concentration of sodium chloride at each equilibrium point. If you instead dialyzed a single time against 1900 ml of phosphate bufor the same total volume of buffer) without transferring what would the equilibrium concentration of sodium chloride be