Read the following sentences and give your opinion on each if you agree or disagree - The factors used to choose a sustainable (green) process are the energy and raw material used and the waste disposal - The transformation or alteration of animal cells from normal to infinite growth capacity can be done by chemical mutagenesis or by fusion with cancerous cells - When mammalian cell cultures grow at high glutamine concentrations and low glucose concentrations, the pH of the medium increases due to ammonia production - High cell and product concentrations in mammalian cell culture can be obtained in fed batch culture with hybridoma cell lines - Metagenomic is the modification of the genome of known microorganisms to produce more biocatalyst - Stagnant zones dominate in bioreactors with Non-Newtonien fluid. The smaller the bioreactor the more homogenuous is the system - The most known antibiotic producers among prokaryotes are Actinomycetes - Warburg effect in mammalian cell culture is equivalent to the pastuer effect in microbial culture - The major advantage of REED is the prevention of membrane fauling - Microbes that rely on anaerobic respiration are very slow grower - To obtain a high cell density E. coli in a fed batch cultures, only glucose and not the whole medium is used in the feeding stream - In the DOE screening experiment, it is advisable to choose the level range of your media components as wide as possible, otherwise the noise will be greater than the signal effect of the tested component - Placket Burmann investigates three levels for each medium components and the best level is then chosen - Comparative proteomic can be done to investigate the intracellular protein profile of an organism under a specific growth condition (only one condition) - To conduct a GSMN, manually curation and filling the gaps in pathways is the second step after automatically generating a first initial draft - For mathematical representation of metabolic network, dynamic modelling is one choice. However, identifying the parameters and constants for each metabolic reaction in a pathway is really a big challenge