Reagents: -Deionized water and a clean anion free plastic washing bottle; -Beer samples: -2 liters of eluent: 12.0mM sodium carbonate 5.0mM Sodium bicarbonate. -10mL of 240mM sodium carbonate 100mM sodium bicarbonate. -Standard anion solutions 1000 mg/L (chloride, fluride, sulphate as S, nitrate as N, nitrite as N and phosphate as P) -Combined working standard solutions Standard preparation: Calculate how to prepare 100.0 mL of a combined anion secondary standard solution with the following concentrations (The concentration of the primary standards for each individual anion was 1000 mg/L as per element form)

-Calculate how you will prepare 25 mL of each standard according to the concentrations listed. You decide the suitable concentrations for STD2.

3- CI (mg/L) 125 SO2? (as S) (mg/L) 50 NO; (as N) (mg/L) 50 NO, (as N) (mg/L) 50 PO2 (as P) (mg/L) 50 ID ? CH SO42--S NO3--N NO2--N PO43--P STD3 (mg/L) STD2(mg/L) STD1(mg/L) 25 ? 1.25 10 ? 0.5 10 ? 0.5 10 ? 0.5 10 ? 0.5 3- CI (mg/L) 125 SO2? (as S) (mg/L) 50 NO; (as N) (mg/L) 50 NO, (as N) (mg/L) 50 PO2 (as P) (mg/L) 50 ID ? CH SO42--S NO3--N NO2--N PO43--P STD3 (mg/L) STD2(mg/L) STD1(mg/L) 25 ? 1.25 10 ? 0.5 10 ? 0.5 10 ? 0.5 10 ? 0.5