Real Life Application P450 enzymes are a ubiquitous superfamily of multifunctional oxidases with important catalytic roles including the metabolism of xenobiotics, such as drugs and environmental pollutants, and biosynthesis of steroids and fat-soluble vitamins. The catalytic mechanism for P450 is typically described by four steps based on the state of the heme iron and oxygen as shown in Scheme 1. It is the reduction of the heme iron-O2 complex by the P450 reductase enzyme using NADPH that starts the reaction cycle by forming the hydroperoxo species and eventually reactive ferryl-oxo T- cation porphyrin radical complex, referred to as compound I. OH N- O=0 Fell EN N-IN| H+ NINH Felli Felil EN NAN -HON Cys Cys Cys Peroxo-ferri Hydroperoxo-ferri Compound Cys Oxy-ferrous NH Cys = . * Cysteinate HO O O HO Heme 16. If BHT were reacted with CrO3 and acetic acid, Draw the product 17. What organic reagent could you use with BHT to get the following oxidized product? 18. If ethanol was added to CYP2E1, and NADPH in the presence of oxygen what would be the product