• Review the alignments, can the sequence difference between the white and red yeast strain be attributed to the difference in the base sequence of ADE1or ADE2.
• Identify the type of mutation present in the coding sequence and discuss the implications of the protein. 
• DNA sequence numbered 60 to 903 (picture 1)
• Protein sequence numbered 60 to 299 (picture 2)
• White coding sequence: White Yeast 

  Red coding sequence: red yeast 

  ADE1: gene

  Dash marks in the sequence represent deletion or mutation.  

• Nucleotide and protein changes from comparisons of white and red ADE1
  
  

WhitecodingADE1 RedcodingADE1 ATGTCAATTACGAAGACTGAACTGGACGGTATATTGCCATTGGTGGCCAGAGGIAAAGTT 60 ATGTCAATTACGAAGACTGAACTGGACGGTATAT CATTGGTGGCCAGAGGTAAAGSTT 60 *****. ....*. WhitecodingADE1 RedcodingADE1 AGAGACATATATGAGGTAGACGCTGGTAC TACGGATCGTATCTCT 120 AGAGACATATATGAGGTAGACGCIGGTACO TGCTGTTTGTTGCTACGGATCGTATCTCT 120 WhitecodingADE1 RedcodingADE1 GCATATGACGTTATTATGGAAA reCTATTGACCAAACTG 180 GCATATGACGITATTATGGAAAACAGCA crGAAAAGGGGATCCTATTGACCAAACTG 180 .***** WhitecodingADE1 RedcodingADE1 TCAGAGTTCTGGTTCAAGTTCCTGTO GTTCG CATTTGGTCGACATCGCC 240 TCAGAGTTCTGGTICAAGITCCTGTcc GATGTTCGTAATCATTTGGTCGACATCGCC 240 WhitecodingADE1 RedcodingADE1 CCAGGIAAGACTATTTTCGATTATCTACCTSCAAAATTGAGCGAACCAAAGTACAAAACG 300 CCAGGIAAGACTATTTTCGATTATCTACCTGCAAAATTGAGCGAACCAAAGTACAAAACG 300 WhitecodingADE1 RedcodingADE1 CAACTAGAAGACCGCTCTCTATTGGTICACAAACATAAACTAATTCCATTGGAAGTAATT 360 CAACTAGAAGACCGCTCTCTATTGGTICACAAACATAAACTAATTCCATTGGAAGTAATT ******** 360 WhitecodingADE1 RedcodingADE1 GTCAGAGGCTACATCACCGGATCTGCTTGGAAAGAGTACGTAAAAACAGGTACTGTGCAT 420 GTCAGAGGCTACATCACCGGATCTGerrGGAAAGAGTACCTAAAAACAGGTCTSTGCAT 420 WhitecodingADE1 RedcodingADE1 GGTTTGAAACAACCTCAAGGACTTAAAGAATCTCAAGAGTTCCCAGAACCAATCTTCACC 480 GGTTTGAAACAACCTCAAGGACTTAAAGAATCTCAAGAGTTCCCAGAACCAATCTTCACC 480 WhitecodingADE1 RedcodingADE1 CCATCGACCAAGGCTGAACAAGGTGAACATGA Gccccr 540 CCATCGACCAAGGCTGAACAAGGTGAACATGACGAAM rererecracccAGGCccCr 540 WhitecodingADE1 RedcodingADE1 GAGCTGGTGGGTGAAGATTTGICACGTAGAGTGGCAGAACTGGCTGTAAAACTGTACTCC 600 GAGCTGGTGGGTGAAGATTTGICACGTAGAGTGGCAGAACTGGCTGTAAAACTGTACTCC ***. 600 660 WhitecodingADE1 RedcodingADE1 AAGTGCAAAGATTATGCTAAGGAGAAGGGCATCA ATCGCAGACACTAAATTCGAATTC AAGTGCAAAGATTATGCTAAGGAGAAGGGCATCATCATCCCAGACACTAAATTCGAATTc 660 ************** ****** WhitecodingADE1 RedcodingADE1 GGTATTGACGAAAAGACCAATGAAATTATICTAGIGGACGAGGTGCTAACGCCAGACTCc 720 GGZATTGACGAAAAGACCAATGAAATTATTCTAGIGGACGAGGTGCTAACGCCAGACTCC 720 ****. **.. ******* ......*. WhitecodingADE1 RedcodingADE1 TCZAGATTCTGGAACGGTGCCTCTTATAAGGTAGGAGAATCCCAAGATTCTTACGATAAG 780 TCTAGATTCTGGAACGGTGCCICTTATAAGGTAGGAGAATCCCAAGATTCITACGATAAG 780 WhitecodingADE1 RedcodingADE1 CAATTITTAAGAGACTGGCTTACTGCTAATAAGTIGAACGGTGTTAACGGCGT-CAMAAT 839 CAATTITTAAGAGACTGGCCTACTGCTAATAAGTTGAACGGTGTTAGCGGCCTCAAAAT 840 ..**. .... ***** WhitecodingADE1 RedcodingADE1 GCCCCAAGACATTGTCGACAGGACAAGGGCCAAATATATAGAGGCITATGAAACATTGAC 899 GCCCCAAGACATTGTCGACAAAAAAGGGC-CATATATATGGGGC-CCATGAAACATTGAT 898 ******************** *... ********.. ************ WhitecodingADE1 RedcodingADE1 AGGGTCTAAATGGICTCACTAA AGAAA- 903 ..