Question
Rewrite and summarise the two text below and explain why is it important? 1. Procedure for Using BD GolgiStop Protein Transport Inhibitor (contains monensin) Add
1. Procedure for Using BD GolgiStop™ Protein Transport Inhibitor (contains monensin) Add 4 µL of BD GolgiStop™ for every 6 mL of cell culture and mix thoroughly. It is recommended that BD GolgiStop not be kept in cell culture for longer than 12 hours. Note: It is recommended that kinetic studies be performed to determine the optimal incubation time for each experimental system.
2. Procedure for Using BD GolgiPlug™ Protein Transport Inhibitor (contains Brefeldin A) BD GolgiPlug™ contains DMSO which is a solid at 4°C. Be sure to thaw at room temperature prior to use. Add 1 µL of BD GolgiPlug for every 1 mL of cell culture and mix thoroughly. It is recommended that BD GolgiPlug not be kept in cell culture for longer than 12 hours. Note: It is recommended that kinetic studies be performed to determine the optimal incubation time for each experimental system.
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