Ribonuclease was used by Christian Anfinsen to study protein folding. What general principle did he discover? The amino acid sequence of a protein specifies its three-dimensional conformation. Disulfide bonds between consecutive cysteine residues determine its tertiary structure. Protein folding involves thermodynamically independent (noncooperative) interactions. The hydrophobic effect driving protein folding is maximized in aqueous solutions containing 8M urea. Question 11 All of the following diseases are associated with misfolded proteins except: Alzheimer's disease Lou Gehrig's disease Parkinson's disease Mad Cow disease