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Sarah wants to measure Methylene Blue dye in the wastewater. In order to measure the MB concentration using UV-Vis Spectrometer, she needs to prepare a
Sarah wants to measure Methylene Blue dye in the wastewater. In order to measure the MB concentration using UV-Vis Spectrometer, she needs to prepare a serial dilution of synthetic Methylene Blue. The concentration of MB in the wastewater sample will be obtained from the absorbance curve plotted. The data is tabulated in Table 1. For dilution purposes, the volume of working concentration, V1 and volume of volumetric flask, V2 can be calculated using the equation: (20 marks) Dilution Equation, C1V1=C2V2 Table 1: Absorbances of Methylene Blue (MB) at Various Concentrations Write the MATLAB command in command windows to determine the following: a) Assign the values for MB working concentration and weight to variables Cl and W b) Sarah would like to prepare extra working solution with C2,0.0081% (w/v). Calculate the volume of working solution 2 to prepare new solution using 50mL volumetric flask. c) If Sarah repeated the dilution in (b) using all the working solutions, which working solution did Sarah used the smallest and largest amount to prepare the 0.0081%(w/v) solution? d) What is the total weight of MB dye used in the working solutions? e) Calculate the average weight of MB dye used for overall working solutions. f) If Sarah would like to prepare a new set of 7 working solution between 0.1%(w/v) to 0.0015%(w/v), what is the new set of dilution data? Use linspace function. Sarah needs to prepare curve graph for absorbance calibration. Plot Absorbance vs MB working concentration (in log10 ) for absorbance calibration with blue dash line and round marker. You don't need to include the title and legend. Use absorbance data below. Table 2: Absorbances of Methylene Blue (MB) at Various Concentrations
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