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show work plz Use the following information and table to answer Questions #1-3 TAE (Tris-Acetic acid-EDTA) is used in gel electrophoresis, as both the solvent
show work plz
Use the following information and table to answer Questions #1-3 TAE (Tris-Acetic acid-EDTA) is used in gel electrophoresis, as both the solvent for the gel and as the electrophoresis buffer (running buffer). Gel electrophoresis is a staple technique in a molecular biology lab, so it's good practice to always have plenty of TAE available. The formula for making a 50x stock solution of TAE is below: 50x TAE stock solution Component Amount Concentration Tris base 242.28 Glacial acetic acid 57.1 ml 500 mM EDTA (pH 8.0) 100000 ul dh,0 to IL 1. What is the molar concentration of Tris in 50x TAE? In IX TAE? (FW Tris base = 121 g/mol) 2. What is the molar concentration of EDTA in 50x TAE? In 1x TAE? 3. You need to make 4 L of Ix TAE. What volume (in ml.) of 50x TAE do you use? 4. A restriction enzyme is supplied at 20,000 units/mL. How many units are present in 10 pal Step by Step Solution
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