So I performed this lab where we used 3 empty plastic water bottles and covered them in black wool craft felt to act as an isolator. we kept 3 bottles (control) without the felt and 3 bottles with the felt all under a heat lamp for 10 minutes. We recorded the initial temp and recorded the internal temperatures every 2 minutes. Then after the 10 minutes under the heat lamp were over we then let the bottles return to normal temp and placed the same six bottles in an ice bath and repeated the steps for data collection. I just need help analyzing my data graphs and t-test, and how it is effective as an insulator mimicking animal fur or pelage.

P U! E I] a. I E 2 4 6 a 1o 12 341.5 ' . E" 1 I A I m " I- n -1.5 . E y=-D.2286x+0.1429 gz R==o.9143 I "--..I .25 Time Figure 1: Line graph depicting time vs internal temp Exposure condition: 3 water bottles covered in Felt under heat lamp {control} a 3 _1 a 4 6 a 10 12 E 2 3 '5' 3 . % " 4 I a 5 I\"' E as y=-o.7235xn.1905 ~1- .. R==o.955a " s 7 .V'I 4; Time Figure 2: Line graph depicting time vs internal temp Exposure condition: 3 water bottles covered in Felt under heat lamp {Experimental} DE 25 20 Internal Tempature [c) 15 10 y- 1.58571 + 9.2381 EEGVO H 12 1 6 10 Time Figure 3: Line graph depicting time vs internal temp Exposure- 3 water bottles covered in felt ice Bath (control]DE 25 2 1857x + 2.9048 20 8 09018 Internal Tempature (c) 15 10 5 2 10 12. Time Figure 4: Line graph depicting time vs internal temp Exposure- 3 water bottles covered in felt in Ice Bath (Experimental)6 Temperature (C) 0 Control Experimental Figure 5 Bar graph depicting the averages of internal temperature for bottles covered in felt under heat lamp condition O -19.5 -20 Control Experimental -20.5 -21 Temperature (c) O -21.5 -22 -22.5 -23 Figure 6 Bar graph depicting the averages of internal temperature for bottles covered in felt in ice bath conditionTHEROMOREGULATION - MODEL ORGANISMS SUPPLIES Supply List SUPPLY LIST Plastic bottles (6 per group) "Pelage' IR Thermometer DAY 1 - ASSIGNED BY INSTRUCTOR Standard Alcohol Thermometer (6 per group) None heat lamp Yoga Mat ice bath White Felt Fan & spray bottle Black Felt BACKGROUND Relevant information for this lab can be found in Chapters 10 and 7, 8 of the text. Students are expected to be very familiar with information from these chapters before the lab. Information in the background section is provided as a refresher, not an exhaustive resource One of the most basic challenges an animal faces is to maintain a biologically relevant body temperature. The function of chemical reactions, proteins and lipids degrade outside this range, impairing an animal's ability to function. This makes temperature homeostasis key to survival, reproduction and passing on genes to the next generation. One way an animal can enhance its thermoregulatory capacity is changing the color of its skin or the covering on top of the skin. Pelage is the term often used to refer to fur, feathers and the like. We will investigate the impact of different colors and different types of pelage in today's lab. Pelage acts to insulate the animal from the outside world. The form of insulation (or lack thereof) depends on the ecological requirements of each organism as well as their evolutionary history- The rationale behind insulation can be explained in part by the heat flux between the animal and environment as described by Fourier's Law. Q = MAT /L A= thermal conductivity of pelage . AT = temperature gradient between inside and outside or organism . L= distance over which AT gradient extends Q= heat transfer The plastic bottles serve as model organisms. This enables us to manipulate environmental conditions rawr! and pelage without having to maintain a large animal collection. It would be hard to shove a lizard in a wetsuit. IMPORTANT: Once pelage is wet, it will not be possible to dry it during the lab period. Keep this in mind when designing your experiments. im a land shark!FIGURE 1. DEMONSTRATION OF BOTTLE CONFIGURATION USING ONLY 1 CONTROL. YOU WILL USE 3 CONTROLS. REQUIRED CALCULATIONS, GRAPHS, AND STATISTICAL TESTS CALCULATIONS FOR T TEST AND GRAPHS - Every 2 minutes calculate how much the internal temperature has changed AT. Subtract the initial temperature (Time=0) from the current temperature. These data are used to make Temperature vs Time line graphs. -At Time=10 minutes, calculate AT for each bottle. Subtract the internal temperature at 10 minutes from the initial bottle temperature. These data are used for the t-tests and bar graphs. FOR EACH EXPOSURE CONDITION Graph: Line graph of Time vs. Internal Temp. -Graph: Bar Graph of average internal temperature for each condition. Use the Group the To-Tap into exposure conditions and types. Average the values. Show error bars on the graph (standard error). - Analyze data using a t-test. Your data points come from the T10 - Tinitial calculation for each bottle. CALCULATE Q Calculate O for one control bottle and one experimental bottle. Use the difference between the water/air temp and the internal temp @T10. L will be the thickness of the yoga mat (or felt or fur). HOW TO SET UP DATA FOR A T-TEST Internal Bottle Temp Internal Bottle Temp Experiment controls AT= To-T 10 AT= To-10 2 10 2 16 wt-Test: Two-Sample Assuming Equal Variances Variable 1 Variable 2 Mean 15 2 Variance 1 Observati 3 3 Pooled Va 0.5 Hypothesi 0 Heat lamp condition df 4 t Stat 22.5166605 P(T