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Lesson Objectives - Perform serial dilutions to prepare solutions of specified concentrations from a stock solution - Be able to accurately use a micropipette. Introduction In this exercise, you will systematically dilute solutions of food dye with water. You will observe how the color intensity, or saturation, of each subsequent solution changes as it becomes more dilute. You will calculate the concentration of each of the diluted solutions. You will also check the accuracy of your pipetting skills by developing a "secret code" in a microtiter culture plate. Experimental Steps to prepare a 1:10 serial dilution Part l: each pair prepare their own blue stock solution (Use a measuring cylinder to measure out 10ml of distilled water, and pour into a beaker. Add two drops of blue food dye. Mix). Keep this stock solution for part II. 1) Refer to figure below. Set up 5 test tubes in a rack. Label them A-E with marker. 2) Pipette 9ml of water into tubes BE. 3) Transfer 2ml of blue stock solution to tube A. 4) Using a P1000 micropipette, transfer 1ml (1000ul) from tube A to B. Mix well. Dispose tip. 5) Using a new tip, transfer 1ml from B to C, and so on as in the figure, each time mixing welf and changing the tip. 6) Fill in the table for part 1 (refer to data sheet). 7) Serial dilutions are multiplicative, so to find the overall (total) dillution in a tube, simply multiply the dilution ratios for each step. - Explain your work for this table: (10 pts) If tube E was diluted the same way one more time to make tube F, what is the final dilution factor for tube F ? Show your full working. ( 5pts ) - How do you construct a 1:100 dilution series? Explain and diagram. (5 pts) 1) Explain why this is a 1:2 dilution. (1 pt) Consider the Serial dilution given next, and complete the table below: (4 pts) 1) What type of Serial dilution is this one (?? :?? dilution)? (1 pt)