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SSPE (Saline/Sodium Phosphate/EDTA) is a commonly used hybridization buffer. It is used at 1X but the stock solution is usually made at 20X. The components
SSPE (Saline/Sodium Phosphate/EDTA) is a commonly used hybridization buffer. It is used at 1X but the stock solution is usually made at 20X. The components of this buffer at 1X are 120.0 mMmM NaCl, 11.0 mMmM sodium phosphate (NaH2PO4) and 1.2 mMmM ethylenediametetraacetic acid (EDTA, disodium salt).
MW (NaCl) = 58.44 g/mol
MW (NaH2PO4) = 119.98 g/mol
MW (EDTA, disodium salt) = 372.24 g/mol What is the molar concentration of NaCl at 20X?
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